1900

Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

[FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.

Abstract

[FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.

Funding source: The European Research Council; Swedish Energy Agency; KA Wallenberg Foundation; Wenner-Gren foundations; NordForsk NCoE program NordAqua
Related subjects: Production & Supply Chain
Countries: Portugal ; Sweden
Loading

Article metrics loading...

/content/journal1350
2018-09-25
2021-08-01
http://instance.metastore.ingenta.com/content/journal1350
Loading

Supplements

Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error