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Chitosan Flocculation Associated with Biofilms of C. Saccharolyticus and C. Owensensis Enhances Biomass Retention in a CSTR


Cell immobilization and co-culture techniques have gained attention due to its potential to obtain high volumetric hydrogen productivities (QH2). Chitosan retained biomass in the fermentation of co-cultures of Caldicellulosiruptor saccharolyticus and C. owensensis efficiently, up to a maximum dilution rate (D) of 0.9 h−1. Without chitosan, wash out of the co-culture occurred earlier, accompanied with approximately 50% drop in QH2 (D > 0.4 h−1). However, butyl rubber did not show as much potential as carrier material; it did neither improve QH2 nor biomass retention in continuous culture. The population dynamics revealed that C. owensensis was the dominant species (95%) in the presence of chitosan, whereas C. saccharolyticus was the predominant (99%) during cultivation without chitosan. In contrast, the co-culture with rubber as carrier maintained the relative population ratios around 1:1. This study highlighted chitosan as an effective potential carrier for immobilization, thereby paving the way for cost – effective hydrogen production.

Funding source: Ministry of Higher Education, Science, Research and Innovation (Thai Government) and the Swedish Research Council Formas (Sweden) (project no. 2017-00795).
Related subjects: Production & Supply Chain
Countries: Sweden

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